› 5us8

X-ray diffraction
2.15Å resolution

2.15 Angstrom Resolution Crystal Structure of Argininosuccinate Synthase from Bordetella pertussis

Released:
DOI: 10.2210/pdb5us8/pdb
PDB entry 5us8 coloured by chain, front view.
PDB entry 5us8 coloured by chain, side view.
PDB entry 5us8 coloured by chain, top view.
Source organism: Bordetella pertussis Tohama I
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Winsor J, Stam J, Kwon K, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182075 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 49.65 KDa
Source organism: Bordetella pertussis Tohama I
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7VTJ9 (Residues: 1-445; Coverage: 100%)
Gene names: BP3537, argG
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand EPE 3 x EPE
Ligand PGE 1 x PGE
Ligand ADN 1 x ADN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P64
Unit cell:
a: 94.453Å b: 94.453Å c: 188.49Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.156 0.2
Expression system: Escherichia coli BL21(DE3)

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