› 5ung

X-ray diffraction
2.8Å resolution

XFEL structure of human angiotensin II type 2 receptor (Orthorhombic form) in complex with compound 1 (N-benzyl-N-(2-ethyl-4-oxo-3-{[2'-(2H-tetrazol-5-yl)[1,1'-biphenyl]-4-yl] methyl}-3,4-dihydroquinazolin-6-yl)thiophene-2-carboxamide)

Released:
DOI: 10.2210/pdb5ung/pdb
PDB entry 5ung coloured by chain, front view.
PDB entry 5ung coloured by chain, side view.
PDB entry 5ung coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for selectivity and diversity in angiotensin II receptors.
Zhang H, Han GW, Batyuk A, Ishchenko A, White KL, Patel N, Sadybekov A, Zamlynny B, Rudd MT, Hollenstein K, Tolstikova A, White TA, Hunter MS, Weierstall U, Liu W, Babaoglu K, Moore EL, Katz RD, Shipman JM, Garcia-Calvo M, Sharma S, Sheth P, Soisson SM, Stevens RC, Katritch V, Cherezov V
Nature 544 327-332 (2017)
PMID: 28379944

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142101 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562; Type-2 angiotensin II receptor Chain: B
Molecule details ›
Chain: B
Length: 411 amino acids
Theoretical weight: 46.51 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: P50052 (Residues: 35-335; Coverage: 83%)
Gene names: AGTR2, cybC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand 8ES 1 x 8ES
Ligand OLC 1 x OLC
Ligand OLA 1 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Unit cell:
a: 70.26Å b: 78.79Å c: 93.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.262
Expression system: Spodoptera frugiperda

Quick links

Citations

32 review citations

Angiotensin II Signal Transduction: An Update on Mechanisms of Physiology and Pathophysiology.
Forrester et al. (2018)
31 more

19 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
18 more