› 5ue1

X-ray diffraction
1.14Å resolution

Crystal structure of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase in complex with adenine from Vibrio fischeri ES114

Released:
DOI: 10.2210/pdb5ue1/pdb
PDB entry 5ue1 coloured by chain, front view.
PDB entry 5ue1 coloured by chain, side view.
PDB entry 5ue1 coloured by chain, top view.
Source organism: Aliivibrio fischeri ES114
Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Dubrovska I, Grimshaw S, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176996 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 24.88 KDa
Source organism: Aliivibrio fischeri ES114
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5E2X3 (Residues: 1-231; Coverage: 100%)
Gene names: VF_2128, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

5 bound ligands:
Ligand 9DA 2 x 9DA
Ligand TRS 2 x TRS
Ligand CA 3 x CA
Ligand CL 5 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 55.642Å b: 72.915Å c: 57.563Å
α: 90° β: 109.6° γ: 90°
R-values:
R R work R free
0.131 0.129 0.158
Expression system: Escherichia coli BL21(DE3)

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