› 5tef

X-ray diffraction
1.95Å resolution

Crystal structure of Gemin5 WD40 repeats in complex with m7GpppG

Released:
DOI: 10.2210/pdb5tef/pdb
PDB entry 5tef coloured by chain, front view.
PDB entry 5tef coloured by chain, side view.
PDB entry 5tef coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.
Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y, Yamauchi Y, Shahjee HM, Wu XH, Yu YT, Isobe T, Takahashi N, Min J
Genes Dev 30 2376-2390 (2016)
PMID: 27881600

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gem-associated protein 5 Chain: A
Molecule details ›
Chain: A
Length: 758 amino acids
Theoretical weight: 84.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TEQ6 (Residues: 1-739; Coverage: 49%)
Gene name: GEMIN5
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

3 bound ligands:
Ligand GTG 1 x GTG
Ligand GOL 3 x GOL
Ligand UNX 19 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 58.658Å b: 124.862Å c: 60.938Å
α: 90° β: 117.52° γ: 90°
R-values:
R R work R free
0.181 0.179 0.217
Expression system: Spodoptera frugiperda

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Citations

7 review citations

Insights into Structural and Mechanistic Features of Viral IRES Elements.
Martinez-Salas et al. (2017)
6 more

2 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
1 more