› 5tad

X-ray diffraction
2.09Å resolution

Conformational Sampling Differences across the Arrhenius Plot Biphasic Break Point at Ambient Temperature in the Enzyme Thermolysin

Released:
DOI: 10.2210/pdb5tad/pdb
PDB entry 5tad coloured by chain, front view.
PDB entry 5tad coloured by chain, side view.
PDB entry 5tad coloured by chain, top view.
Source organism: Bacillus thermoproteolyticus
Entry authors: Dong M, Bahnson BJ

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -|-Leu > -|-Phe.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CA 4 x CA
Ligand ZN 1 x ZN
Ligand VAL 1 x VAL
Ligand LYS 1 x LYS
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6122
Unit cell:
a: 93.779Å b: 93.779Å c: 131.076Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.147 0.197
Expression system: Bacillus subtilis

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