› 5olv

X-ray diffraction
2Å resolution

Structure of the A2A-StaR2-bRIL562-LUAA47070 complex at 2.0A obtained from in meso soaking experiments.

Released:
DOI: 10.2210/pdb5olv/pdb
PDB entry 5olv coloured by chain, front view.
PDB entry 5olv coloured by chain, side view.
PDB entry 5olv coloured by chain, top view.
Source organisms:
Primary publication:
Towards high throughput GPCR crystallography: In Meso soaking of Adenosine A2A Receptor crystals.
Rucktooa P, Cheng RKY, Segala E, Geng T, Errey JC, Brown GA, Cooke RM, Marshall FH, Doré AS
Sci Rep 8 41 (2018)
PMID: 29311713

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine receptor A2a; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 48.07 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P29274 (Residues: 2-208, 219-317; Coverage: 74%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: ADORA2, ADORA2A, cybC
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand 9Y2 1 x 9Y2
Ligand NA 1 x NA
Ligand CLR 4 x CLR
Ligand OLA 13 x OLA
Ligand OLC 10 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 39.429Å b: 180.774Å c: 140.903Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.208
Expression system: Trichoplusia ni

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Citations

7 review citations

Targeting adenosine A2A receptor antagonism for treatment of cancer.
Congreve et al. (2018)
6 more

12 mentions without citation

Harnessing Ion-Binding Sites for GPCR Pharmacology.
Zarzycka et al. (2019)
11 more