› 5o4n

X-ray diffraction
2.05Å resolution

Apo HcgC from Methanococcus maripaludis soaked with SAH and pyridinol

Released:
DOI: 10.2210/pdb5o4n/pdb
PDB entry 5o4n coloured by chain, front view.
PDB entry 5o4n coloured by chain, side view.
PDB entry 5o4n coloured by chain, top view.
Source organism: Methanococcus maripaludis S2
Primary publication:
A Water-Bridged H-Bonding Network Contributes to the Catalysis of the SAM-Dependent C-Methyltransferase HcgC.
Bai L, Wagner T, Xu T, Hu X, Ermler U, Shima S
Angew Chem Int Ed Engl 56 10806-10809 (2017)
PMID: 28682478

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HcgC Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 274 amino acids
Theoretical weight: 30.94 KDa
Source organism: Methanococcus maripaludis S2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6LX54 (Residues: 1-260; Coverage: 100%)
Gene name: MMP1498
Sequence domains: FeGP cofactor biosynthesis protein, methyltransferase HcgC

Ligands and Environments

4 bound ligands:
Ligand SAH 4 x SAH
Ligand 9KH 4 x 9KH
Ligand MPD 5 x MPD
Ligand DMS 4 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 72.556Å b: 80.997Å c: 97.808Å
α: 90° β: 108.24° γ: 90°
R-values:
R R work R free
0.199 0.197 0.236
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Green Rust: The Simple Organizing 'Seed' of All Life?
Russell MJ. (2018)
2 other articles