› 5n7l

X-ray diffraction
2.5Å resolution

Crystal structure of the periplasmic domain of XcpY, tI crystal form.

Released:
DOI: 10.2210/pdb5n7l/pdb
PDB entry 5n7l coloured by chain, front view.
PDB entry 5n7l coloured by chain, side view.
PDB entry 5n7l coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
Fulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN
Sci Rep 8 16760 (2018)
PMID: 30425318

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein L Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 8.11 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25060 (Residues: 303-382; Coverage: 21%)
Gene names: PA3096, xcpY
Sequence domains: GspL periplasmic domain

Ligands and Environments

2 bound ligands:
Ligand BR 3 x BR
Ligand GLU 3 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: I4122
Unit cell:
a: 100.439Å b: 100.439Å c: 98.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Architecture, Function, and Substrates of the Type II Secretion System.
Korotkov et al. (2019)
2 more

1 mention without citation

Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
Fulara et al. (2018)