› 5my4

X-ray diffraction
2.21Å resolution

Structure of Pyroglutamate-Abeta-specific Fab c#17 in complex with human Abeta-pE3-12PEGb

Released:
DOI: 10.2210/pdb5my4/pdb
PDB entry 5my4 coloured by chain, front view.
PDB entry 5my4 coloured by chain, side view.
PDB entry 5my4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analyses of pyroglutamate-amyloid-β-specific antibodies as a basis for Alzheimer immunotherapy.
Piechotta A, Parthier C, Kleinschmidt M, Gnoth K, Pillot T, Lues I, Demuth HU, Schilling S, Rahfeld JU, Stubbs MT
J Biol Chem 292 12713-12724 (2017)
PMID: 28623233

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209167 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab c#17 light chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.08 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab c#17 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 231 amino acids
Theoretical weight: 24.65 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Amyloid-beta protein 40 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 674-683; Coverage: 1%)
Gene names: A4, AD1, APP

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 43.17Å b: 87.142Å c: 58.029Å
α: 90° β: 96.06° γ: 90°
R-values:
R R work R free
0.189 0.186 0.25
Expression systems:
  • Mus musculus
  • Not provided

Quick links

Citations

2 review citations

The Amyloid-β Oligomer Hypothesis: Beginning of the Third Decade.
Cline et al. (2018)
1 more

1 mention without citation

Structural and functional analyses of pyroglutamate-amyloid-β-specific antibodies as a basis for Alzheimer immunotherapy.
Piechotta et al. (2017)