› 5mlz

X-ray diffraction
2Å resolution

Dolichyl phosphate mannose synthase in complex with GDP and Mg2+

Released:
DOI: 10.2210/pdb5mlz/pdb
PDB entry 5mlz coloured by chain, front view.
PDB entry 5mlz coloured by chain, side view.
PDB entry 5mlz coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structural basis for dolichylphosphate mannose biosynthesis.
Gandini R, Reichenbach T, Tan TC, Divne C
Nat Commun 8 120 (2017)
PMID: 28743912

Function and Biology Details

Reaction catalysed: 
GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichol-phosphate mannosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 42.68 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M3 (Residues: 1-352; Coverage: 100%)
Gene name: PF0058
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand LDA 7 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 90.87Å b: 146.21Å c: 95.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.257
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nucleotide Sugars in Chemistry and Biology.
Mikkola S. (2020)
3 more

7 mentions without citation

Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces.
Allen et al. (2019)
6 more