› 5mke

Electron Microscopy
4.3Å resolution

cryoEM Structure of Polycystin-2 in complex with cations and lipids

Released:
DOI: 10.2210/pdb5mke/pdb
PDB entry 5mke coloured by chain, front view.
PDB entry 5mke coloured by chain, side view.
PDB entry 5mke coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular insights into lipid-assisted Ca2+ regulation of the TRP channel Polycystin-2.
Wilkes M, Madej MG, Kreuter L, Rhinow D, Heinz V, De Sanctis S, Ruppel S, Richter RM, Joos F, Grieben M, Pike AC, Huiskonen JT, Carpenter EP, Kühlbrandt W, Witzgall R, Ziegler C
Nat Struct Mol Biol 24 123-130 (2017)
PMID: 28092368
Related structures: EMD-3523

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Polycystin-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 968 amino acids
Theoretical weight: 109.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13563 (Residues: 1-968; Coverage: 100%)
Gene names: PKD2, TRPP2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand NAG 10 x NAG
Ligand CHS 8 x CHS
Ligand PX6 4 x PX6
Ligand PLM 12 x PLM
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-3523
Expression system: Homo sapiens

Quick links

Citations

19 review citations

Polycystins, ADPKD, and Cardiovascular Disease.
Kuo et al. (2020)
18 more

9 mentions without citation

Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3.
Hirschi et al. (2017)
8 more