› 5mdq

X-ray diffraction
2.5Å resolution

Crystal structure of outer membrane expressed Chitoporin VhChip from Vibrio harveyi

Released:
DOI: 10.2210/pdb5mdq/pdb
PDB entry 5mdq coloured by chain, front view.
PDB entry 5mdq coloured by chain, side view.
PDB entry 5mdq coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Structural basis for chitin acquisition by marine Vibrio species.
Aunkham A, Zahn M, Kesireddy A, Pothula KR, Schulte A, Baslé A, Kleinekathöfer U, Suginta W, van den Berg B
Nat Commun 9 220 (2018)
PMID: 29335469

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 350 amino acids
Theoretical weight: 38.54 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: L0RVU0 (Residues: 26-375; Coverage: 100%)
Gene name: chiP
Sequence domains: Gram-negative porin

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 255.73Å b: 148.639Å c: 54.364Å
α: 90° β: 94.94° γ: 90°
R-values:
R R work R free
0.23 0.23 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.
Horne et al. (2020)
1 more

5 mentions without citation

Structural basis for chitin acquisition by marine Vibrio species.
Aunkham et al. (2018)
4 more