› 5m87

X-ray diffraction
3.3Å resolution

Crystal structure of Eremococcus coleocola manganese transporter

Released:
DOI: 10.2210/pdb5m87/pdb
PDB entry 5m87 coloured by chain, front view.
PDB entry 5m87 coloured by chain, side view.
PDB entry 5m87 coloured by chain, top view.
Source organism: Eremococcus coleocola ACS-139-V-Col8
Primary publication:
Structural and mechanistic basis of proton-coupled metal ion transport in the SLC11/NRAMP family.
Ehrnstorfer IA, Manatschal C, Arnold FM, Laederach J, Dutzler R
Nat Commun 8 14033 (2017)
PMID: 28059071

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Divalent metal cation transporter MntH Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 56.63 KDa
Source organism: Eremococcus coleocola ACS-139-V-Col8
Expression system: Escherichia coli
UniProt:
  • Canonical: E4KPW4 (Residues: 1-511; Coverage: 100%)
Gene names: HMPREF9257_1603, mntH
Sequence domains: Natural resistance-associated macrophage protein-like

Ligands and Environments

1 bound ligand:
Ligand DMU 1 x DMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 149.2Å b: 81.65Å c: 96.23Å
α: 90° β: 107.57° γ: 90°
R-values:
R R work R free
0.234 0.232 0.277
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.
Fairweather et al. (2021)
5 more

9 mentions without citation

Molecular Mechanism of Nramp-Family Transition Metal Transport.
Bozzi et al. (2021)
8 more