› 5lue

Solution NMR

Minor form of the recombinant cytotoxin-1 from N. oxiana

Released:
DOI: 10.2210/pdb5lue/pdb
PDB entry 5lue coloured by chain, ensemble of 20 models, front view.
PDB entry 5lue coloured by chain, ensemble of 20 models, side view.
PDB entry 5lue coloured by chain, ensemble of 20 models, top view.
Source organism: Naja oxiana
Primary publication:
Structural and Dynamic "Portraits" of Recombinant and Native Cytotoxin I from Naja oxiana: How Close Are They?
Dubovskii PV, Dubinnyi MA, Konshina AG, Kazakova ED, Sorokoumova GM, Ilyasova TM, Shulepko MA, Chertkova RV, Lyukmanova EN, Dolgikh DA, Arseniev AS, Efremov RG
Biochemistry 56 4468-4477 (2017)
PMID: 28749688

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytotoxin 1 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.96 KDa
Source organism: Naja oxiana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01451 (Residues: 1-14, 16-60; Coverage: 98%)
Structure domains: CD59

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: restrained Rosetta minimization
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The role of hydrophobic /hydrophilic balance in the activity of structurally flexible vs. rigid cytolytic polypeptides and analogs developed on their basis.
Dubovskii et al. (2018)
2 other articles

1 mention without citation

Membrane-Disrupting Activity of Cobra Cytotoxins Is Determined by Configuration of the N-Terminal Loop.
Dubovskii et al. (2022)