› 5ls0

X-ray diffraction
1.83Å resolution

Crystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb5ls0/pdb
PDB entry 5ls0 coloured by chain, front view.
PDB entry 5ls0 coloured by chain, side view.
PDB entry 5ls0 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structures of plant inorganic pyrophosphatase, an enzyme with a moonlighting autoproteolytic activity.
Grzechowiak M, Ruszkowski M, Sliwiak J, Szpotkowski K, Sikorski M, Jaskolski M
Biochem J 476 2297-2319 (2019)
PMID: 31371393

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble inorganic pyrophosphatase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 20.52 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93V56 (Residues: 30-208; Coverage: 84%)
Gene names: At1g01050, PPA1, T25K16.5
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: R3
Unit cell:
a: 81.13Å b: 81.13Å c: 174.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.154 0.19
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Magnesium Signaling in Plants.
Kleczkowski et al. (2021)
5 other articles

1 mention without citation

Crystal structure of an inorganic pyrophosphatase from Chlamydia trachomatis D/UW-3/Cx.
Maddy et al. (2022)