› 5kx5

X-ray diffraction
2.5Å resolution

Crystal structure of tubulin-stathmin-TTL-Compound 11 complex

Released:
DOI: 10.2210/pdb5kx5/pdb
PDB entry 5kx5 coloured by chain, front view.
PDB entry 5kx5 coloured by chain, side view.
PDB entry 5kx5 coloured by chain, top view.
Source organisms:
Primary publication:
Design, Synthesis, and Cytotoxic Evaluation of Novel Tubulysin Analogues as ADC Payloads.
Leverett CA, Sukuru SC, Vetelino BC, Musto S, Parris K, Pandit J, Loganzo F, Varghese AH, Bai G, Liu B, Liu D, Hudson S, Doppalapudi VR, Stock J, O'Donnell CJ, Subramanyam C
ACS Med Chem Lett 7 999-1004 (2016)
PMID: 27882198

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-236839 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.19 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.97 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 16.85 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
TTL protein Chain: F
Molecule details ›
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 6 x MG
Ligand CA 4 x CA
Ligand GDP 2 x GDP
Ligand 6YK 2 x 6YK
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 104.79Å b: 153.9Å c: 185.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.251
Expression system: Escherichia coli

Quick links

Citations

3 review citations

DNA damaging agent-based antibody-drug conjugates for cancer therapy.
Fu et al. (2018)
2 more

1 mention without citation

Development of DNA aptamers for visualization of glial brain tumors and detection of circulating tumor cells.
Kichkailo et al. (2023)