› 5kuj

X-ray diffraction
1.6Å resolution

Human mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium.

Released:
DOI: 10.2210/pdb5kuj/pdb
PDB entry 5kuj coloured by chain, front view.
PDB entry 5kuj coloured by chain, side view.
PDB entry 5kuj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into Mitochondrial Calcium Uniporter Regulation by Divalent Cations.
Lee SK, Shanmughapriya S, Mok MCY, Dong Z, Tomar D, Carvalho E, Rajan S, Junop MS, Madesh M, Stathopulos PB
Cell Chem Biol 23 1157-1169 (2016)
PMID: 27569754

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium uniporter protein, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NE86 (Residues: 72-189; Coverage: 34%)
Gene names: C10orf42, CCDC109A, MCU
Sequence domains: Mitochondrial calcium uniporter

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P65
Unit cell:
a: 55.36Å b: 55.36Å c: 68.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.199
Expression system: Escherichia coli

Quick links

Citations

20 review citations

The Involvement of Mg2+ in Regulation of Cellular and Mitochondrial Functions.
Pilchova et al. (2017)
19 more

6 mentions without citation

Mitochondrial Ca2+ transport in the endothelium: regulation by ions, redox signalling and mechanical forces.
Alevriadou et al. (2017)
5 more