› 5kr5

X-ray diffraction
2.1Å resolution

Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins for New Chemistries

Released:
DOI: 10.2210/pdb5kr5/pdb
PDB entry 5kr5 coloured by chain, front view.
PDB entry 5kr5 coloured by chain, side view.
PDB entry 5kr5 coloured by chain, top view.
Source organism: Pseudomonas
Primary publication:
Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction
Wilding M, Peat TS, Kalyaanamoorthy S, Newman J, Scott C, Jermiin LS
Green Chemistry 19 5375- (2017)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-aminobutyrate transaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 479 amino acids
Theoretical weight: 52.03 KDa
Source organism: Pseudomonas
Expression system: Escherichia coli BL21
Structure domains: Type I PLP-dependent aspartate aminotransferase-like (Major domain)

Ligands and Environments

3 bound ligands:
Ligand PLP 2 x PLP
Ligand CA 3 x CA
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 98.451Å b: 102.538Å c: 199.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.24
Expression system: Escherichia coli BL21

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