› 5kkh

X-ray diffraction
2.12Å resolution

2.1-Angstrom In situ Mylar structure of bacteriorhodopsin from Haloquadratum walsbyi (HwBR) at 100 K

Released:
DOI: 10.2210/pdb5kkh/pdb
PDB entry 5kkh coloured by chain, front view.
PDB entry 5kkh coloured by chain, side view.
PDB entry 5kkh coloured by chain, top view.
Source organism: Haloquadratum walsbyi DSM 16790
Primary publication:
A Versatile System for High-Throughput In Situ X-ray Screening and Data Collection of Soluble and Membrane-Protein Crystals.
Broecker J, Klingel V, Ou WL, Balo AR, Kissick DJ, Ogata CM, Kuo A, Ernst OP
Cryst Growth Des 16 6318-6326 (2016)
PMID: 28261000

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin-I Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 268 amino acids
Theoretical weight: 29.12 KDa
Source organism: Haloquadratum walsbyi DSM 16790
Expression system: Escherichia coli
UniProt:
  • Canonical: Q18DH8 (Residues: 1-254; Coverage: 100%)
Gene names: HQ_1014A, bop1, bopI
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand OLC 6 x OLC
Ligand OLB 3 x OLB
Ligand RET 3 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 106.11Å b: 61.27Å c: 119.03Å
α: 90° β: 116.26° γ: 90°
R-values:
R R work R free
0.214 0.213 0.233
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs.
Broecker et al. (2017)
7 more