› 5kji

X-ray diffraction
2.71Å resolution

Crystal structure of an active polycomb repressive complex 2 in the basal state

Released:
DOI: 10.2210/pdb5kji/pdb
PDB entry 5kji coloured by chain, front view.
PDB entry 5kji coloured by chain, side view.
PDB entry 5kji coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
Response to Comment on "Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2".
Jiao L, Liu X
Science 354 1543 (2016)
PMID: 28008038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123899 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
putative polycomb protein Eed Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 66.02 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: G0S8H7 (Residues: 1-565; Coverage: 100%)
Gene name: CTHT_0029920
Sequence domains: WD domain, G-beta repeat
Polycomb protein VEFS-Box domain-containing protein; SET domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 937 amino acids
Theoretical weight: 106.87 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: G0SDW4 (Residues: 191-950; Coverage: 50%)
  • Canonical: G0RYC6 (Residues: 530-691; Coverage: 22%)
Gene names: CTHT_0006210, CTHT_0053230
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 82.333Å b: 74.619Å c: 127.035Å
α: 90° β: 105.63° γ: 90°
R-values:
R R work R free
0.183 0.179 0.249
Expression system: Saccharomyces cerevisiae S288C

Quick links

Citations

2 review citations

PRC2 is high maintenance.
Yu et al. (2019)
1 more

5 mentions without citation

Structure, mechanism, and regulation of polycomb-repressive complex 2.
Moritz et al. (2018)
4 more