› 5k97

X-ray diffraction
2.1Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118900 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Flap endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39748 (Residues: 2-336; Coverage: 88%)
Gene names: FEN1, RAD2
Sequence domains:
Structure domains:
DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 11 nucleotides
Theoretical weight: 3.44 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*CP*CP*GP*TP*CP*C)-3') Chain: F
Molecule details ›
Chain: F
Length: 7 nucleotides
Theoretical weight: 2.06 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 5 nucleotides
Theoretical weight: 1.48 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand SM 8 x SM
Ligand K 1 x K
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P3121
Unit cell:
a: 105.236Å b: 105.236Å c: 104.548Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.219
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair.
Syed et al. (2018)
10 more

5 mentions without citation

Phosphate steering by Flap Endonuclease 1 promotes 5'-flap specificity and incision to prevent genome instability.
Tsutakawa et al. (2017)
4 more