› 5jka

X-ray diffraction
2Å resolution

Crystal structure of human JUNO (crystal form 1)

Released:
DOI: 10.2210/pdb5jka/pdb
PDB entry 5jka coloured by chain, front view.
PDB entry 5jka coloured by chain, side view.
PDB entry 5jka coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization.
Ohto U, Ishida H, Krayukhina E, Uchiyama S, Inoue N, Shimizu T
Nature 534 566-9 (2016)
PMID: 27309808

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sperm-egg fusion protein Juno Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.43 KDa
Source organism: Homo sapiens
Expression system: Drosophila
UniProt:
  • Canonical: A6ND01 (Residues: 20-228; Coverage: 91%)
Gene names: FOLR4, IZUMO1R, JUNO
Sequence domains: Folate receptor family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: I212121
Unit cell:
a: 51.92Å b: 81.038Å c: 235.084Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.218
Expression system: Drosophila

Quick links

Citations

23 review citations

The hallmarks of cell-cell fusion.
Hernández et al. (2017)
22 more

1 mention without citation

Molecular dynamics of JUNO-IZUMO1 complexation suggests biologically relevant mechanisms in fertilization.
Pacak et al. (2023)