› 5jjj

X-ray diffraction
2.5Å resolution

Structure of the SRII/HtrII Complex in P64 space group ("U" shape)

Released:
DOI: 10.2210/pdb5jjj/pdb
PDB entry 5jjj coloured by chain, front view.
PDB entry 5jjj coloured by chain, side view.
PDB entry 5jjj coloured by chain, top view.
Source organism: Natronomonas pharaonis
Primary publication:
New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex.
Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Remeeva A, Polovinkin V, Utrobin P, Balandin T, Engelhard M, Büldt G, Gordeliy V
Sci Rep 7 41811 (2017)
PMID: 28165484

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154503 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sensory rhodopsin-2 Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 26.53 KDa
Source organism: Natronomonas pharaonis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42196 (Residues: 2-239; Coverage: 100%)
Gene names: sop2, sopII
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins
Sensory rhodopsin II transducer Chain: B
Molecule details ›
Chain: B
Length: 141 amino acids
Theoretical weight: 14.76 KDa
Source organism: Natronomonas pharaonis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42259 (Residues: 5-137; Coverage: 25%)
Gene names: htr2, htrII
Sequence domains:
Structure domains: Helix hairpin bin

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand LFA 8 x LFA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P64
Unit cell:
a: 66.31Å b: 66.31Å c: 170.081Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.233
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural insights into the signalling mechanisms of two-component systems.
Jacob-Dubuisson et al. (2018)
4 more

2 mentions without citation

New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex.
Ishchenko et al. (2017)
1 more