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X-ray diffraction
2.34Å resolution

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2B4 Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 54.48 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00178 (Residues: 20-491; Coverage: 96%)
Gene name: CYP2B4
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand 32M 1 x 32M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3121
Unit cell:
a: 91.165Å b: 91.165Å c: 151.137Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.265
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Relationship between Hepatitis C Virus Infection and Iron Overload.
Zou et al. (2017)
2 more

1 mention without citation

Effect of detergent binding on cytochrome P450 2B4 structure as analyzed by X-ray crystallography and deuterium-exchange mass spectrometry.
Shah et al. (2016)