› 5iqt

X-ray diffraction
2.4Å resolution

WelO5 bound to Fe(II), Cl, 2-oxoglutarate, and 12-epifischerindole U

Released:
DOI: 10.2210/pdb5iqt/pdb
PDB entry 5iqt coloured by chain, front view.
PDB entry 5iqt coloured by chain, side view.
PDB entry 5iqt coloured by chain, top view.
Source organism: Hapalosiphon welwitschii UTEX B 1830
Primary publication:
Structural basis for halogenation by iron- and 2-oxo-glutarate-dependent enzyme WelO5.
Mitchell AJ, Zhu Q, Maggiolo AO, Ananth NR, Hillwig ML, Liu X, Boal AK
Nat Chem Biol 12 636-40 (2016)
PMID: 27348090

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WelO5 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 315 amino acids
Theoretical weight: 35.53 KDa
Source organism: Hapalosiphon welwitschii UTEX B 1830
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A067YX61 (Residues: 1-290; Coverage: 100%)

Ligands and Environments

4 bound ligands:
Ligand FE2 3 x FE2
Ligand CL 3 x CL
Ligand AKG 3 x AKG
Ligand 6CU 2 x 6CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 71.045Å b: 83.067Å c: 141.944Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.234
Expression system: Escherichia coli

Quick links

Citations

15 review citations

2-Oxoglutarate-Dependent Oxygenases.
Islam et al. (2018)
14 more

5 mentions without citation

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
4 more