› 5iou

Electron Microscopy
7Å resolution

Cryo-EM structure of GluN1/GluN2B NMDA receptor in the glutamate/glycine-bound conformation

Released:
DOI: 10.2210/pdb5iou/pdb
PDB entry 5iou coloured by chain, front view.
PDB entry 5iou coloured by chain, side view.
PDB entry 5iou coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Mechanism of NMDA Receptor Inhibition and Activation.
Zhu S, Stein RA, Yoshioka C, Lee CH, Goehring A, Mchaourab HS, Gouaux E
Cell 165 704-14 (2016)
PMID: 27062927
Related structures: EMD-8097

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103597 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutamate receptor ionotropic, NMDA 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 822 amino acids
Theoretical weight: 92.65 KDa
Source organism: Xenopus laevis
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A1L8F5J9 (Residues: 23-836; Coverage: 91%)
Gene name: grin1
Sequence domains:
Glutamate receptor ionotropic, NMDA 2B Chains: B, D
Molecule details ›
Chains: B, D
Length: 825 amino acids
Theoretical weight: 93.23 KDa
Source organism: Xenopus laevis
Expression system: Homo sapiens
UniProt:
  • Canonical: A7XY94 (Residues: 1-839; Coverage: 56%)
Gene name: grin2b
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GLY 2 x GLY
Ligand GLU 2 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7Å
Relevant EMDB volumes: EMD-8097
Expression system: Homo sapiens

Quick links

Citations

21 review citations

Structure, function, and allosteric modulation of NMDA receptors.
Hansen et al. (2018)
20 more

10 mentions without citation

Structure and symmetry inform gating principles of ionotropic glutamate receptors.
Zhu et al. (2017)
9 more