› 5inf

X-ray diffraction
2.75Å resolution

Structural basis for acyl-CoA carboxylase-mediated assembly of unusual polyketide synthase extender units incorporated into the stambomycin antibiotics

Released:
DOI: 10.2210/pdb5inf/pdb
PDB entry 5inf coloured by chain, front view.
PDB entry 5inf coloured by chain, side view.
PDB entry 5inf coloured by chain, top view.
Source organism: Streptomyces ambofaciens ATCC 23877
Primary publication:
A crotonyl-CoA reductase-carboxylase independent pathway for assembly of unusual alkylmalonyl-CoA polyketide synthase extender units.
Ray L, Valentic TR, Miyazawa T, Withall DM, Song L, Milligan JC, Osada H, Takahashi S, Tsai SC, Challis GL
Nat Commun 7 13609 (2016)
PMID: 28000660

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative propionyl-CoA carboxylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 538 amino acids
Theoretical weight: 58.1 KDa
Source organism: Streptomyces ambofaciens ATCC 23877
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0ACI9 (Residues: 1-532; Coverage: 100%)
Gene names: SAM23877_7108, SAMR0483
Sequence domains: Carboxyl transferase domain
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand HXC 1 x HXC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 110.072Å b: 165.453Å c: 190.261Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Engineered polyketides: Synergy between protein and host level engineering.
Barajas et al. (2017)
1 more

1 mention without citation

A crotonyl-CoA reductase-carboxylase independent pathway for assembly of unusual alkylmalonyl-CoA polyketide synthase extender units.
Ray et al. (2016)