› 5iga

X-ray diffraction
1.45Å resolution

Crystal structure of a marine metagenome TRAP solute binding protein specific for aromatic acid ligands (Sorcerer II Global Ocean Sampling Expedition, unidentified microbe, locus tag GOS_1523157, Triple Surface Mutant K158A_K223A_K313A) in complex with co-purified parahydroxybenzoate

Released:
DOI: 10.2210/pdb5iga/pdb
PDB entry 5iga coloured by chain, front view.
PDB entry 5iga coloured by chain, side view.
PDB entry 5iga coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Vetting MW, Al Obaidi NF, Hogle SL, Dupont CL, Almo SC

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP TRANSPORTER SOLUTE BINDING PROTEIN Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 37.48 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

3 bound ligands:
Ligand PEG 5 x PEG
Ligand TRS 1 x TRS
Ligand PHB 1 x PHB
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 39.83Å b: 84.47Å c: 46.069Å
α: 90° β: 113.22° γ: 90°
R-values:
R R work R free
0.121 0.12 0.143
Expression system: Escherichia coli BL21(DE3)

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