› 5hv9

X-ray diffraction
3Å resolution

Human LTC4S mutant-S36E

Released:
DOI: 10.2210/pdb5hv9/pdb
PDB entry 5hv9 coloured by chain, front view.
PDB entry 5hv9 coloured by chain, side view.
PDB entry 5hv9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs Catalytic Activity.
Ahmad S, Ytterberg AJ, Thulasingam M, Tholander F, Bergman T, Zubarev R, Wetterholm A, Rinaldo-Matthis A, Haeggström JZ
J Biol Chem 291 18410-8 (2016)
PMID: 27365393

Function and Biology Details

Reaction catalysed: 
Leukotriene C(4) = leukotriene A(4) + glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leukotriene C4 synthase Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.45 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q16873 (Residues: 2-150; Coverage: 99%)
Gene name: LTC4S
Sequence domains: MAPEG family
Structure domains: Membrane associated eicosanoid/glutathione metabolism-like domain

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: F23
Unit cell:
a: 168.042Å b: 168.042Å c: 168.042Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.289 0.312
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Leukotriene biosynthetic enzymes as therapeutic targets.
Haeggström JZ. (2018)
1 more

1 mention without citation

Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs Catalytic Activity.
Ahmad et al. (2016)