› 5hnw

Electron Microscopy
6.6Å resolution

Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the AMPPNP state

Released:
DOI: 10.2210/pdb5hnw/pdb
PDB entry 5hnw coloured by chain, front view.
PDB entry 5hnw coloured by chain, side view.
PDB entry 5hnw coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of Backwards Motion in Kinesin-1-Kinesin-14 Chimera: Implication for Kinesin-14 Motility.
Yamagishi M, Shigematsu H, Yokoyama T, Kikkawa M, Sugawa M, Aoki M, Shirouzu M, Yajima J, Nitta R
Structure 24 1322-1334 (2016)
PMID: 27452403
Related structures: EMD-8058

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-148990 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 49.98 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 2-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 444 amino acids
Theoretical weight: 49.78 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 2-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin heavy chain isoform 5C; Protein claret segregational Chain: K
Molecule details ›
Chain: K
Length: 371 amino acids
Theoretical weight: 41.62 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P20480 (Residues: 325-349, 664-700; Coverage: 9%)
  • Canonical: P56536 (Residues: 10-320; Coverage: 32%)
Gene names: CA(ND), CG7831, Kif5c, Nkhc2, ncd
Sequence domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-8058
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Molecular mechanisms of kinesin-14 motors in spindle assembly and chromosome segregation.
She et al. (2017)
4 more

1 mention without citation

New tools for the analysis and validation of cryo-EM maps and atomic models.
Afonine et al. (2018)