› 5hjc

X-ray diffraction
2.6Å resolution

BRD3 second bromodomain in complex with histone H3 acetylation at K18

Released:
DOI: 10.2210/pdb5hjc/pdb
PDB entry 5hjc coloured by chain, front view.
PDB entry 5hjc coloured by chain, side view.
PDB entry 5hjc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Coupling of Histone Crotonylation and Active Transcription by AF9 YEATS Domain.
Li Y, Sabari BR, Panchenko T, Wen H, Zhao D, Guan H, Wan L, Huang H, Tang Z, Zhao Y, Roeder RG, Shi X, Allis CD, Li H
Mol Cell 62 181-193 (2016)
PMID: 27105114

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159408 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain-containing protein 3 Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 13.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15059 (Residues: 307-416; Coverage: 15%)
Gene names: BRD3, KIAA0043, RING3L
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 16-24; Coverage: 7%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand CL 1 x CL
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 79.768Å b: 79.768Å c: 95.262Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.217 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

62 review citations

Metabolic regulation of gene expression through histone acylations.
Sabari et al. (2017)
61 more

1 mention without citation

Molecular Coupling of Histone Crotonylation and Active Transcription by AF9 YEATS Domain.
Li et al. (2016)