› 5hif

X-ray diffraction
1.6Å resolution

Crystal structure of a reconstructed lactonase ancestor, Anc1-MPH, of the bacterial methyl parathion hydrolase, MPH.

Released:
DOI: 10.2210/pdb5hif/pdb
PDB entry 5hif coloured by chain, front view.
PDB entry 5hif coloured by chain, side view.
PDB entry 5hif coloured by chain, top view.
Source organism: synthetic construct
Entry authors: Baier F, Carr PD, Jackson CJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165736 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
reconstructed lactonase ancestor, Anc1-MPH Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 31.52 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand PEG 2 x PEG
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Xenocs GeniX 3D Cu HF
Spacegroup: P212121
Unit cell:
a: 57.6Å b: 88.15Å c: 119.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links