5gzn

X-ray diffraction
3Å resolution

Structure of neutralizing antibody bound to Zika envelope protein

Released:

Function and Biology Details

Reactions catalysed:
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-234897 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Genome polyprotein Chains: A, B, E, G
Molecule details ›
Chains: A, B, E, G
Length: 409 amino acids
Theoretical weight: 44.61 KDa
Source organism: Zika virus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1B0XTC8 (Residues: 291-699; Coverage: 12%)
Sequence domains:
Structure domains:
Antibody Heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 228 amino acids
Theoretical weight: 24.47 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector pBGSA
Structure domains: Immunoglobulins
Antibody light chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 216 amino acids
Theoretical weight: 22.76 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector pBGSA
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 124.708Å b: 129.385Å c: 428.711Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.258
Expression systems:
  • Escherichia coli
  • Mammalian expression vector pBGSA