› 5g4i

X-ray diffraction
1.5Å resolution

PLP-dependent phospholyase A1RDF1 from Arthrobacter aurescens TC1

Released:
DOI: 10.2210/pdb5g4i/pdb
PDB entry 5g4i coloured by chain, front view.
PDB entry 5g4i coloured by chain, side view.
PDB entry 5g4i coloured by chain, top view.
Source organism: Paenarthrobacter aurescens TC1
Primary publication:
Structural Basis for Phospholyase Activity of a Class III Transaminase Homologue.
Cuetos A, Steffen-Munsberg F, Mangas Sanchez J, Frese A, Bornscheuer UT, Höhne M, Grogan G
Chembiochem 17 2308-2311 (2016)
PMID: 27709756

Function and Biology Details

Reaction catalysed: 
Ethanolamine phosphate + H(2)O = acetaldehyde + NH(3) + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative Aminotransferase class III protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 48.09 KDa
Source organism: Paenarthrobacter aurescens TC1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1RDF1 (Residues: 1-446; Coverage: 100%)
Gene name: AAur_pTC20101
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand PO4 5 x PO4
Ligand ACT 3 x ACT
Ligand B3P 1 x B3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 76.937Å b: 97.176Å c: 124.261Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Structural and kinetic characterization of (S)-1-amino-2-propanol kinase from the aminoacetone utilization microcompartment of Mycobacterium smegmatis.
Mallette et al. (2018)
1 more

1 mention without citation

The Arabidopsis thaliana gene annotated by the locus tag At3g08860 encodes alanine aminotransferase.
Parthasarathy et al. (2019)