› 5fs8

X-ray diffraction
1.4Å resolution

Crystal structure of the G308E mutant of human apoptosis inducing factor

Released:
DOI: 10.2210/pdb5fs8/pdb
PDB entry 5fs8 coloured by chain, front view.
PDB entry 5fs8 coloured by chain, side view.
PDB entry 5fs8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure/Function Relations in AIFM1 Variants Associated with Neurodegenerative Disorders.
Sevrioukova IF
J Mol Biol 428 3650-65 (2016)
PMID: 27178839

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 56.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95831 (Residues: 103-613; Coverage: 83%)
Gene names: AIF, AIFM1, PDCD8
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 1 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 50.79Å b: 89.75Å c: 60.33Å
α: 90° β: 94.93° γ: 90°
R-values:
R R work R free
0.166 0.164 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Apoptosis-Inducing Factor (AIF) in Physiology and Disease: The Tale of a Repented Natural Born Killer.
Bano et al. (2018)
9 more

3 mentions without citation

FAD/NADH Dependent Oxidoreductases: From Different Amino Acid Sequences to Similar Protein Shapes for Playing an Ancient Function.
Trisolini et al. (2019)
2 more