› 5frd

X-ray diffraction
1.4Å resolution

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 29.29 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28735 (Residues: 1-251; Coverage: 100%)
Gene name: AF_1537
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

5 bound ligands:
Ligand COA 2 x COA
Ligand CL 2 x CL
Ligand PGE 1 x PGE
Ligand FLC 1 x FLC
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 55.07Å b: 67.2Å c: 140.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Structure of the Catalytic Domain of the Class I Polyhydroxybutyrate Synthase from Cupriavidus necator.
Wittenborn et al. (2016)
3 more

4 mentions without citation

Improving the 'tool box' for robust industrial enzymes.
Littlechild JA. (2017)
3 more