› 5fhi

X-ray diffraction
2.41Å resolution

Crystallographic structure of PsoE without Co

Released:
DOI: 10.2210/pdb5fhi/pdb
PDB entry 5fhi coloured by chain, front view.
PDB entry 5fhi coloured by chain, side view.
PDB entry 5fhi coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Oxidative trans to cis Isomerization of Olefins in Polyketide Biosynthesis.
Yamamoto T, Tsunematsu Y, Hara K, Suzuki T, Kishimoto S, Kawagishi H, Noguchi H, Hashimoto H, Tang Y, Hotta K, Watanabe K
Angew Chem Int Ed Engl 55 6207-10 (2016)
PMID: 27072782

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase psoE Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.72 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WB03 (Residues: 1-237; Coverage: 100%)
Gene names: AFUA_8G00580, psoE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GSH 1 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P6522
Unit cell:
a: 145.413Å b: 145.413Å c: 51.266Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.185 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Oxidative Cyclization in Natural Product Biosynthesis.
Tang et al. (2017)
1 more