› 5f8p

X-ray diffraction
2.2Å resolution

A Novel Inhibitor of the Obesity-Related Protein FTO

Released:
DOI: 10.2210/pdb5f8p/pdb
PDB entry 5f8p coloured by chain, front view.
PDB entry 5f8p coloured by chain, side view.
PDB entry 5f8p coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Chai J, Zhou B, Liu W, Han Z

Function and Biology Details

Reaction catalysed: 
N(6)-methyladenosine in mRNA + 2-oxoglutarate + O(2) = adenosine in mRNA + formaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutarate-dependent dioxygenase FTO Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 54.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C0B1 (Residues: 32-502; Coverage: 93%)
Gene names: FTO, KIAA1752
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 1 x SO4
Ligand FIJ 1 x FIJ
Ligand AKG 1 x AKG
Ligand FE2 1 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 142.218Å b: 142.218Å c: 83.028Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.234
Expression system: Escherichia coli

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