› 5f7s

X-ray diffraction
2.3Å resolution

Cycloalternan-degrading enzyme from Trueperella pyogenes

Released:
DOI: 10.2210/pdb5f7s/pdb
PDB entry 5f7s coloured by chain, front view.
PDB entry 5f7s coloured by chain, side view.
PDB entry 5f7s coloured by chain, top view.
Source organism: Trueperella pyogenes
Primary publication:
Structure to function of an α-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis.
Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF
Nat Microbiol 2 16202 (2016)
PMID: 27819654

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 31 Chains: A, B
Molecule details ›
Chains: A, B
Length: 733 amino acids
Theoretical weight: 82.33 KDa
Source organism: Trueperella pyogenes
Expression system: Escherichia coli BL21
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 9 x CA
Ligand PEG 1 x PEG
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 197.126Å b: 104.663Å c: 89.03Å
α: 90° β: 90.74° γ: 90°
R-values:
R R work R free
0.172 0.17 0.211
Expression system: Escherichia coli BL21

Quick links

Citations

15 citation in other articles

A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria.
Light et al. (2018)
14 more

4 mentions without citation

New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag.
Biancucci et al. (2017)
3 more