› 5ev0

X-ray diffraction
2.1Å resolution

Crystal structure of ragweed profilin Amb a 8 in complex with poly-Pro14

Released:
DOI: 10.2210/pdb5ev0/pdb
PDB entry 5ev0 coloured by chain, front view.
PDB entry 5ev0 coloured by chain, side view.
PDB entry 5ev0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2.
Offermann LR, Schlachter CR, Perdue ML, Majorek KA, He JZ, Booth WT, Garrett J, Kowal K, Chruszcz M
J Biol Chem 291 15447-59 (2016)
PMID: 27231348

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165621 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Profilin Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 14.35 KDa
Source organism: Ambrosia artemisiifolia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2KN24 (Residues: 2-133; Coverage: 99%)
Gene names: M8C21_011050, M8C21_011055
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic
PRO-PRO-PRO-PRO-PRO-PRO-PRO-PRO-PRO Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 892 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 55.132Å b: 40.342Å c: 60.256Å
α: 90° β: 104.42° γ: 90°
R-values:
R R work R free
0.195 0.192 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe.
Chen et al. (2018)
2 more

5 mentions without citation

Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability.
Booth et al. (2018)
4 more