› 5efc

X-ray diffraction
1.9Å resolution

Structure of Influenza B Lee PB2 cap-binding domain bound to GTP

Released:
DOI: 10.2210/pdb5efc/pdb
PDB entry 5efc coloured by chain, front view.
PDB entry 5efc coloured by chain, side view.
PDB entry 5efc coloured by chain, top view.
Source organism: Influenza B virus (B/Lee/1940)
Primary publication:
Molecular Basis of mRNA Cap Recognition by Influenza B Polymerase PB2 Subunit.
Xie L, Wartchow C, Shia S, Uehara K, Steffek M, Warne R, Sutton J, Muiru GT, Leonard VH, Bussiere DE, Ma X
J Biol Chem 291 363-70 (2016)
PMID: 26559973

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.44 KDa
Source organism: Influenza B virus (B/Lee/1940)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QLL6 (Residues: 319-485; Coverage: 22%)
Gene name: PB2
Sequence domains: Influenza RNA polymerase PB2 CAP binding domain

Ligands and Environments

1 bound ligand:
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 40.515Å b: 46.93Å c: 87.952Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Influenza Virus Polymerase Complex: An Update on Its Structure, Functions, and Significance for Antiviral Drug Design.
Stevaert et al. (2016)
4 more

3 mentions without citation

Molecular Basis of mRNA Cap Recognition by Influenza B Polymerase PB2 Subunit.
Xie et al. (2016)
2 more