› 5e9s

X-ray diffraction
2.8Å resolution

Crystal structure of substrate-bound glutamate transporter homologue GltTk

Released:
DOI: 10.2210/pdb5e9s/pdb
PDB entry 5e9s coloured by chain, front view.
PDB entry 5e9s coloured by chain, side view.
PDB entry 5e9s coloured by chain, top view.
Source organism: Thermococcus kodakarensis
Primary publication:
Coupled binding mechanism of three sodium ions and aspartate in the glutamate transporter homologue GltTk.
Guskov A, Jensen S, Faustino I, Marrink SJ, Slotboom DJ
Nat Commun 7 13420 (2016)
PMID: 27830699

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton/glutamate symporter, SDF family Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 438 amino acids
Theoretical weight: 46.69 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JID0 (Residues: 1-430; Coverage: 100%)
Gene name: TK0986
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

7 bound ligands:
Ligand ASP 3 x ASP
Ligand NA 9 x NA
Ligand DMU 3 x DMU
Ligand 1PE 7 x 1PE
Ligand PEG 33 x PEG
Ligand PGE 7 x PGE
Ligand P6G 5 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 117.52Å b: 117.52Å c: 310.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.213 0.242
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Advances and Challenges in Rational Drug Design for SLCs.
Garibsingh et al. (2019)
7 more

12 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
11 more