› 5e76

X-ray diffraction
2.3Å resolution

Crystal structure of Bacova_02651 with xylogluco-oligosaccharide

Released:
DOI: 10.2210/pdb5e76/pdb
PDB entry 5e76 coloured by chain, front view.
PDB entry 5e76 coloured by chain, side view.
PDB entry 5e76 coloured by chain, top view.
Source organism: Bacteroides ovatus ATCC 8483
Primary publication:
Molecular Dissection of Xyloglucan Recognition in a Prominent Human Gut Symbiont.
Tauzin AS, Kwiatkowski KJ, Orlovsky NI, Smith CJ, Creagh AL, Haynes CA, Wawrzak Z, Brumer H, Koropatkin NM
mBio 7 e02134-15 (2016)
PMID: 27118585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
SusD-like protein BACOVA_02651 Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 58.27 KDa
Source organism: Bacteroides ovatus ATCC 8483
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7LXT5 (Residues: 36-546; Coverage: 97%)
Gene name: BACOVA_02651
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, XYS
Carbohydrate polymer
3 bound ligands:
Ligand EDO 5 x EDO
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I422
Unit cell:
a: 131.5Å b: 131.5Å c: 188.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Introduction to the human gut microbiota.
Thursby et al. (2017)
6 more

4 mentions without citation

Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
Grondin et al. (2022)
3 more