› 5e5r

X-ray diffraction
2.6Å resolution

Crystal structure of the complex between Carbonic anhydrase-like domain of PTPRG and Immunoglobulin domains 2-3 of CNTN3

Released:
DOI: 10.2210/pdb5e5r/pdb
PDB entry 5e5r coloured by chain, front view.
PDB entry 5e5r coloured by chain, side view.
PDB entry 5e5r coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Interactions Between Contactin Family Members and Protein-tyrosine Phosphatase Receptor Type G in Neural Tissues.
Nikolaienko RM, Hammel M, Dubreuil V, Zalmai R, Hall DR, Mehzabeen N, Karuppan SJ, Harroch S, Stella SL, Bouyain S
J Biol Chem 291 21335-21349 (2016)
PMID: 27539848

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149981 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor-type tyrosine-protein phosphatase gamma Chains: A, C
Molecule details ›
Chains: A, C
Length: 265 amino acids
Theoretical weight: 30.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23470 (Residues: 56-320; Coverage: 19%)
Gene names: PTPG, PTPRG
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase
Contactin-3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 193 amino acids
Theoretical weight: 21.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07409 (Residues: 124-316; Coverage: 19%)
Gene names: Cntn3, Pang, Pcs
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand MLI 3 x MLI
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 74.138Å b: 90.526Å c: 147.448Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.249
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Conserved and Diverse Traits of Adhesion Devices from Siphoviridae Recognizing Proteinaceous or Saccharidic Receptors.
Goulet et al. (2020)
9 more

1 mention without citation

Structural Basis for Interactions Between Contactin Family Members and Protein-tyrosine Phosphatase Receptor Type G in Neural Tissues.
Nikolaienko et al. (2016)