› 5dys

X-ray diffraction
2.3Å resolution

Crystal Structure of T94I rhodopsin mutant

Released:
DOI: 10.2210/pdb5dys/pdb
PDB entry 5dys coloured by chain, front view.
PDB entry 5dys coloured by chain, side view.
PDB entry 5dys coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural role of the T94I rhodopsin mutation in congenital stationary night blindness.
Singhal A, Guo Y, Matkovic M, Schertler G, Deupi X, Yan EC, Standfuss J
EMBO Rep 17 1431-1440 (2016)
PMID: 27458239

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.05 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand RET 1 x RET
Ligand ACT 2 x ACT
Ligand BOG 2 x BOG
Ligand PLM 2 x PLM
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R32
Unit cell:
a: 242.301Å b: 242.301Å c: 112.055Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.219 0.231
Expression system: Homo sapiens

Quick links

Citations

3 review citations

The molecular and cellular basis of rhodopsin retinitis pigmentosa reveals potential strategies for therapy.
Athanasiou et al. (2018)
2 more

8 mentions without citation

Structural Basis for G Protein-Coupled Receptor Activation.
Manglik et al. (2017)
7 more