› 5dqq

X-ray diffraction
2.87Å resolution

Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb5dqq/pdb
PDB entry 5dqq coloured by chain, front view.
PDB entry 5dqq coloured by chain, side view.
PDB entry 5dqq coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana.
Kintzer AF, Stroud RM
Nature 531 258-62 (2016)
PMID: 26961658

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Two pore calcium channel protein 1 Chain: A
Molecule details ›
Chain: A
Length: 723 amino acids
Theoretical weight: 83.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q94KI8 (Residues: 11-733; Coverage: 99%)
Gene names: At4g03560, CCH1, F9H3.19, FOU2, T5L23.5, TPC1
Sequence domains: Ion transport protein

Ligands and Environments

3 bound ligands:
Ligand CA 7 x CA
Ligand PLM 1 x PLM
Ligand 66R 1 x 66R
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1, ALS BEAMLINE 5.0.2, SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 88.18Å b: 154.81Å c: 219.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.299 0.297 0.339
Expression system: Saccharomyces cerevisiae

Quick links

Citations

36 review citations

The chemical basis for electrical signaling.
Catterall et al. (2017)
35 more

12 mentions without citation

The Discovery of Naringenin as Endolysosomal Two-Pore Channel Inhibitor and Its Emerging Role in SARS-CoV-2 Infection.
D'Amore et al. (2021)
11 more