› 5dpl

X-ray diffraction
3.2Å resolution

The structure of PKMT2 from Rickettsia typhi in complex with AdoHcy

Released:
DOI: 10.2210/pdb5dpl/pdb
PDB entry 5dpl coloured by chain, front view.
PDB entry 5dpl coloured by chain, side view.
PDB entry 5dpl coloured by chain, top view.
Source organism: Rickettsia typhi str. Wilmington
Primary publication:
Structural Insights into Substrate Recognition and Catalysis in Outer Membrane Protein B (OmpB) by Protein-lysine Methyltransferases from Rickettsia.
Abeykoon AH, Noinaj N, Choi BE, Wise L, He Y, Chao CC, Wang G, Gucek M, Ching WM, Chock PB, Buchanan SK, Yang DC
J Biol Chem 291 19962-74 (2016)
PMID: 27474738

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 61.4 KDa
Source organism: Rickettsia typhi str. Wilmington
Expression system: Escherichia coli
UniProt:
  • Canonical: Q68XQ5 (Residues: 1-534; Coverage: 100%)
Gene name: RT0101
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 83.352Å b: 91.028Å c: 105.827Å
α: 90° β: 112.3° γ: 90°
R-values:
R R work R free
0.247 0.244 0.28
Expression system: Escherichia coli

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Citations

3 review citations

The enigmatic biology of rickettsiae: recent advances, open questions and outlook.
McGinn et al. (2021)
2 more

2 mentions without citation

Efficient Remyelination Requires DNA Methylation.
Moyon et al. (2017)
1 more