› 5d8f

X-ray diffraction
2.35Å resolution

crystal structure of SSB and ssDNA complex from homo sapiens

Released:
DOI: 10.2210/pdb5d8f/pdb
PDB entry 5d8f coloured by chain, front view.
PDB entry 5d8f coloured by chain, side view.
PDB entry 5d8f coloured by chain, top view.
Source organisms:
Entry authors: Li YH, Gao ZQ, Dong YH

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118544 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
SOSS complex subunit B1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BQ15 (Residues: 1-109; Coverage: 52%)
Gene names: LP3587, NABP2, OBFC2B, SSB1
Sequence domains: Single-stranded DNA binding protein Ssb-like, OB fold
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6422
Unit cell:
a: 83.821Å b: 83.821Å c: 159.315Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.238 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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