› 5cxv

X-ray diffraction
2.7Å resolution

Structure of the human M1 muscarinic acetylcholine receptor bound to antagonist Tiotropium

Released:
DOI: 10.2210/pdb5cxv/pdb
PDB entry 5cxv coloured by chain, front view.
PDB entry 5cxv coloured by chain, side view.
PDB entry 5cxv coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of the M1 and M4 muscarinic acetylcholine receptors.
Thal DM, Sun B, Feng D, Nawaratne V, Leach K, Felder CC, Bures MG, Evans DA, Weis WI, Bachhawat P, Kobilka TS, Sexton PM, Kobilka BK, Christopoulos A
Nature 531 335-40 (2016)
PMID: 26958838

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132963 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endolysin; Muscarinic acetylcholine receptor M1 Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 58.65 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11229 (Residues: 2-218, 227-227, 355-460; Coverage: 70%)
  • Canonical: P00720 (Residues: 2-11, 13-161; Coverage: 97%)
Gene names: CHRM1, E
Sequence domains:
FLAG peptide Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 886 Da
Source organism: Tequatrovirus T4
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand 0HK 1 x 0HK
Ligand Y01 1 x Y01
Ligand EDO 3 x EDO
Ligand PGE 1 x PGE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 58.053Å b: 72.186Å c: 175.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.282
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

40 review citations

Targeting Muscarinic Acetylcholine Receptors for the Treatment of Psychiatric and Neurological Disorders.
Moran et al. (2019)
39 more

63 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
62 more