› 5cjj

X-ray diffraction
2.42Å resolution

The crystal structure of phosphoribosylglycinamide formyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168

Released:
DOI: 10.2210/pdb5cjj/pdb
PDB entry 5cjj coloured by chain, front view.
PDB entry 5cjj coloured by chain, side view.
PDB entry 5cjj coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Tan K, Zhou M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.65 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0PBV2 (Residues: 1-188; Coverage: 100%)
Gene names: Cj0187c, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

4 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
Ligand CL 1 x CL
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 65.162Å b: 65.162Å c: 368.905Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.249 0.29
Expression system: Escherichia coli BL21(DE3)

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